Table 2.
Sensitivity of all algorithms
| Single-tier | STTT | MTTT-strict | MTTT-permissive | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| No. of true positive (sensitivity %) | 95%-CI | No. of true positive (sensitivity %) | 95%-CI | No. of true positive (sensitivity %) | 95%-CI | No. of true positive (sensitivity %) | 95%-CI | |||
| EM sera (n = 228) | ||||||||||
| Enz1 | 180 (78.9)1 | 73.1–84.1 | Enz1/RecomL | 106 (46.5) | 39.9–53.2 | Enz1/C6 | 162 (71.1)1,2 | 64.7–76.8 | 173 (75.9)1,4,5 | 69.8–81.3 |
| Enz2 | 184 (80.7)1 | 75.0–85.6 | Enz2/RecomL | 106 (46.5) | 39.9–53.2 | Enz2/C6 | 166 (72.8)1,2 | 66.5–78.5 | 177 (77.6)1,4,5 | 71.7–82.9 |
| Lia | 160 (70.2)1 | 63.8–76.0 | Lia/RecomL | 101 (44.3) | 37.7–51.0 | Lia/C6 | 153 (67.1)1,2 | 60.6–73.2 | 166 (72.8)1,3,4,5 | 66.5–78.5 |
| C6 | 178 (78.1)* | 72.1–83.3 | C6/RecomL | 102 (44.7) | 38.2–51.4 | |||||
1p < 0.01 as compared with equivalent STTT using (McNemar/exact McNemar) | (*C6 vs. C6/RecomL p = 0.016)
2p < 0.01 as compared with single-tier C6-ELISA (one-sample t test)
3p < 0.01 as compared with single-tier C6 (exact McNemar)
4p < 0.01 as compared with equivalent MTTT-strict (one-sample t test)
5Not significant as compared with equivalent single-tier (exact McNemar)
A test result was considered to be (true) positive when either the IgM component, IgG component or both were positive
95%-CI, 95% confidence interval; STTT, standard two-tier testing; MTTT, modified two-tier testing; Enz1, Enzygnost-1; Enz2, Enzygnost-2; Lia, Liaison; C6, C6-ELISA; RecomL, RecomLine; EM, erythema migrans; PopC, population control; CRC, cross-reactive control; strict, counting equivocal EIA results as negative; permissive, counting equivocal EIA results as positive