Fig. 2.

Diagram of mouse Ntrk2 mRNA variants and protein structure. a Diagram of seven mouse Ntrk2 mRNA variants which encode four isoforms of mouse NTRK2 proteins (821, 476, 536, 492 aa). Translated regions are shown by boxes, and 3′ untranslated regions are shown by colored lines. Box with the same color or shape has the same mRNA sequence. Amino acid number of coded protein segment is shown on top of each box. Colored line with the same color has the same RNA sequence. These seven variants have seven different kinds of 3′UTR. The locations of probes used in FISH experiment are shown in the figure. Probe 1 detects transcripts with 3′UTR-A or 3′UTR-AB. Probe 2 only detects transcript with 3′UTR-AB. Probe 3 detects transcripts with both 3′UTR-C and 3′UTR-CD. Probe 4 only detects transcripts with 3′UTR-CD. Primers used in QPCR are also shown in the picture. b Schematic structure of 821aa mouse NTRK2 protein domains