Table 3.
The sequences of primers used in cloning or RT-PCR
| Purpose | Target | Forward primer | Reverse primer |
|---|---|---|---|
| Cloning |
3′UTR-A of Ntrk2 mRNA |
GGGTCCTCCTTCTGCCCAGACCGTCCTTCC | TAACTTTTTAATGGAGTATAGTTAACAA |
| Cloning |
3′UTR-B of Ntrk2 mRNA |
GAATTAAGCCTTGACACTGTATGGCTGACA | ACCTCTTGTGGAAGATGTTATTTATTG |
| Cloning |
3′UTR-AB of Ntrk2 mRNA |
GGGTCCTCCTTCTGCCCAGACCGTCCTTCC | ACCTCTTGTGGAAGATGTTATTTATTG |
| RT-PCR | β-actin | AAGTCCCTCACCCTCCCAAAAG | AAGCAATGCTGTCACCTTCCC |
| Primer set A RT-PCR | Ntrk2 mRNA with 3′UTR-A or 3′-UTR AB | TGACCAATCTGGCTTCTGCATTCC | GGTGGGCAAACTGGAGTGTCTG |
| Primer set B RT-PCR | Ntrk2 mRNA with 3′UTR AB | GGTACTGTCAGGCACTGTGGAAC | TAGGTCACGGCTGGCGGAAG |
| Primer set C RT-PCR | Ntrk2 mRNA with 3′UTR C or with 3′UTR CD | GCAGGTAGAACGGAGCAGCAC | CAGAGGGCAATGGAAAGGGACAAG |
| Primer set D RT-PCR | Ntrk2 mRNA with 3′UTR D | GGACCGCCATCAGCAACACAG | TCCCTTCTCTCTTCCTGGCACTG |
| Primer se E RT-PCR | Ntrk2 mRNA with 3′UTR-E | CTGACAGCATGGGGTGGTTGAC | GGCAAGGCAAGGAGAAGATAGCAC |
| Primer set F RT-PCR | Ntrk2 mRNA with 3′UTR-F | CAAGACTTGGCGGTGACCTGTG | AGGGAGGCAACATCAGAAAAGACC |