Fig. 2.

LSDV replicates and spreads on MDBK cells. (A) MDBK cells (n = 12) or bovine primary fibroblasts (n = 6) were inoculated with LSDV (MOI = 5). At the timepoints shown cells were scraped into the supernatant and virus titred on MDBK cells. Values shown are means, error bars are SEM. (B and C) MDBK cells were inoculated with LSDV wildtype (B) or LSDV Neethling (C) at a MOI of 5 (one-step growth curve) or MOI = 0.01 (multi-step growth curve). At the timepoints shown virus loads in the cell associated (PEL) and supernatant (SUP) fractions were determined by plaque assay on MDBK cells. Values are the mean of two biological replicates, error bars = SEM.