Figure 5.

Average Contraction Peaks of EHT Drug Responses

Average contraction peaks are depicted at baseline (black) and at the indicated concentration of the respective drug (red). Data were generated in Tyrode's solution with submaximal Ca²⁺ and electrical stimulation. Rows show effects of different drugs, columns separate different hiPSC-CM cell lines. PLU: 0.5 mM calcium, 1 Hz; CDI: 0.6 mM calcium, 1.5 Hz; ICE: 0.6 mM calcium, 1.5 Hz; CEL: 0.5 mM calcium, 0.8 Hz; COR: 1 mM calcium; 3 Hz/2.5 Hz; C25: 0.5 mM calcium, 1.5 Hz/2Hz; AT1: 0.6 mM calcium, 1.5 Hz; NCR: 0.6 mM calcium, 1 Hz; n = 2–6 each; data represent mean ± SEM; for details please see Table S2. Note: NCR data on BayK-8644 do not show electrically stimulated EHT peaks, as prolongation of RT led to failure of capture. Isoprenaline data paced with 1.5 Hz. Note that for visualization, contraction peaks of EHTs that ceased beating (e.g., COR at 0.3 μM digoxin) are not included in average peak illustration but in concentrations response curves (see also Figure S4 and Table S3).