Fig. 4.

Effects of 95AH on the expressions of Bcl-2 and Bax in H₂O₂ treated PC12 cells. Cells were pretreated with the indicated concentrations of 95AH for 24 h and then treated with H₂O₂ for 2 h. Protein levels of Bax and Bcl-2 were analyzed by western blotting using actin as the internal control. (A) Representative protein expressions of Bcl-2 and Bax. (B) Expression level of Bcl-2. (C) Expression level of Bax. (D) Bcl-2/Bax ratio. CTL, control; 95AH, 95% ethanol extract of A. hookeri. The results shown are representative of three independent experiments. Data with different letters represent statistical differences from one another (p<0.05)