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. 2020 Oct 15;13:73. doi: 10.1186/s12284-020-00428-x

Fig. 10.

Fig. 10

Expression analysis of representative genes related to grain shape in Oat-like rice, OsMADS1Olr-overexpressing and OsMADS1-RNAi plants. a-c Relative expression of representative genes related to grain shape in NIP and Oat-like rice plants (a), OsMADS1Olr-overexpressing plants (b) and OsMADS1-RNAi plants (c) by qRT-PCR analysis. For each sample, total RNA isolated from 12 DAF grains was used for qRT-PCR analysis. The value of OsActin mRNA was used as an internal control for data normalization, and the expression levels of genes in NIP were set as 1.0 (a-c). Values are means ± SDs of three replicates. Student’s t-test: *p < 0.05, **p < 0.01, ***p < 0.001