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. 2020 Oct 15;11:5209. doi: 10.1038/s41467-020-18905-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative images are shown for control (left) animals, and animals receiving hemodynamic management with high-dose dobutamine (middle) and norepinephrine (right). Merged stains (top) are shown for ionized calcium binding adaptor molecule 1 (IBA1+, middle) and glial fibrillary acidic protein (GFAP+, bottom). Group data are shown for immunohistochemical analyses of GFAP (b) and IBA1 (c). Bars plots represent means and error bars represent s.d. No significant differences were detected between animals in control (CON), high-dose dobutamine (DOB+), and norepinephrine (NE) groups. n = 4 per group for immunohistochemical analyses. Source data are provided as a Source Data file.