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. 2020 Oct 15;11(10):859. doi: 10.1038/s41419-020-03078-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Protein levels of phosphorylated Smad1/5/8 and Smad1/5/8 in E8.5 non-BMP4 Tg and BMP4 Tg embryos from nondiabetic (ND) or diabetes mellitus (DM) dams. Quantification of relative expression levels of phosphorylation of Smad1/5/8 versus total Samd1/5/8 was shown in the graphs. b Protein levels of Smad4 in E8.5 non-BMP4 Tg and BMP4 Tg embryos from nondiabetic (ND) or diabetes mellitus (DM) dams. Quantification of relative expression levels of Smad4 was shown in the graphs. Experiments were performed using three embryos from three different dams per group (n = 3); * indicates significant difference compared to the other groups (P < 0.05) in one-way ANOVA followed by Tukey tests. ND: nondiabetic; DM: diabetes mellitus.