Skip to main content
. 2020 Oct 15;11:5200. doi: 10.1038/s41467-020-19001-7

Fig. 1. Experimental design and QC of multi-omics datasets of murine in vitro neuronal differentiation.

Fig. 1

a Experimental design of this study. We differentiate mESC towards neural progenitors (NPC48h) in vitro. We treat mESC and NPC for 48 h either with the DOT1L inhibitor EPZ5676 (10 μM) or with DMSO (1/1000) as control. For each sample, we generate transcriptomics data via RNA-seq and comprehensive epigenomes using quantitative ChIP-seq. We further map the accessible chromatin landscape for NPC48h using ATAC-seq. b Upper panel: principal component analysis of the transcriptome of mESC and NPC48h on the top 500 most variable genes (rlog transformed counts) shows a separation between the two cell types on the first principal component. Lower panel: multiple factor analysis of the epigenome of mESC and NPC48h computed over the top 500 most variable 2 kb windows for each histone modification yield similar results. Biological replicates are denoted by the same color.