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. 2020 Oct 15;10:17407. doi: 10.1038/s41598-020-73647-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of K_d values from binding of (A) PT, (B) fX, (C) fIX, (D) fVIIa, (E) aPC, (F) PrS, and (G) PrZ to Nanodiscs with (blue) and without (red) PE lipids. K_d values are plotted as a function of percent PS where the PS + PE lipid composition is equal to 50% for the PE lipid containing Nanodiscs. Each was determined by plotting relative shift of binding vs. concentration of protein and fitting to Eq. (1). Error bars represent the standard deviation from at least n = 4 microrings. K_d values for 50% PE/50% PC are not shown in the graphs due to the large error from the poor binding to this lipid environment by each clotting factor. # aPC binding to 10% PS without PE was too weak to calculate K_d. Plots generated in RStudio 1.1.453 and R 3.5.1 (https://www.R-project.org/) using the ‘ggplot2’ package.