Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 15;10:17448. doi: 10.1038/s41598-020-74491-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Duodenal acid induces tryptase expression, without changing mast cell counts or ultrastructure. Duodenal biopsy samples after saline perfusion (black dots) and after acid perfusion (white dots) were stained for eosinophils using eosinophilic MBP (n = 10 for saline and n = 9 for acid perfusion) (A) and for mast cells using tryptase (n = 9 for both groups) (C). Representative images of MBP (B) and tryptase (D) immunohistochemistry in mucosal biopsy specimens obtained after saline (left) and after acid perfusion (right). Scale bar: 20 µm. (E) Protein expression of MBP and tryptase was measured by western blot after intraduodenal saline (black dots) and acid (white dots) perfusion (n = 10 for both groups). (F) Representative western blot of five saline perfused and five acid perfused subjects. Bands were cropped from different parts of the same gel, or from different gels. (G) Electrodensity of the mast cells granules after saline and acid perfusion. (H) Similar ultrastructure of the duodenal epithelium after saline and acid perfusion showing no changes in cell morphology or integrity of the epithelium (×3000). Data are mean ± SEM; ***P < 0.001. MBP, eosinophilic major basic protein.