Table 1.
mRNA expression of cell-to-cell adhesion proteins and eosinophil and mast cell markers.
| Gene | Saline | Acid | P value |
|---|---|---|---|
| CLDN1 | 1.06 ± 0.14 | 0.93 ± 0.13 | 0.39 |
| CLDN2 | 0.82 (0.65–2.08) | 0.93 (0.57–1.60) | 0.74 |
| CLDN3 | 1.08 (0.87–1.19) | 1.00 (0.76–1.02) | 1.00 |
| CLDN4 | 1.02 ± 0.07 | 1.15 ± 0.13 | 0.27 |
| OCLN | 1.03 ± 0.09 | 0.91 ± 0.09 | 0.29 |
| ZO1 | 1.01 ± 0.05 | 1.07 ± 0.13 | 0.81 |
| ZO2 | 1.02 ± 0.07 | 0.97 ± 0.10 | 0.84 |
| ZO3 | 1.02 ± 0.06 | 0.96 ± 0.12 | 0.83 |
| β-catenin | 1.00 ± 0.03 | 0.93 ± 0.07 | 0.53 |
| E-cadherin | 1.02 ± 0.06 | 0.89 ± 0.12 | 0.46 |
| DSC2 | 1.02 ± 0.06 | 0.95 ± 0.10 | 0.80 |
| DSG2 | 1.05 ± 0.11 | 0.85 ± 0.15 | 0.18 |
| PRG2 | 1.04 ± 0.11 | 0.93 ± 0.08 | 0.81 |
| TPSAB1 | 2.08 ± 0.58 | 1.60 ± 0.56 | 0.20 |
Real-time RT-PCR was used to evaluate the gene expression of cell-to-cell adhesion proteins (upper panel) and an eosinophil marker (PRG2) and a mast cell marker (TPSAB1) (lower panel). Data are mean ± SEM or median (IQR). CLDN, claudin; OCLN, occludin; ZO, zonula occludens; DSC2, desmocollin-2; DSG2, desmoglein-2; PRG2, eosinophil major basic protein; TPSAB1, tryptase Alpha/Beta 1.