Figure 5.

Presence of Diverse RG Cells in COs

(A) Low-power composite image of HOPX, CRYAB, and SOX2 expression in day 140 COs. White frame indicates reference for (B).

(B) High-power merged and single-channel images from inset in (A).

(C) Schematic depicting progenitor zones in the CO model.

(D) Feature UMAPs of integrated glia and proliferative progenitor clusters (note the retained shape of clusters from Figure 3). From top to bottom: expression of aRG-specific markers PALLD, ANXA1, and CRYAB; bRG-specific markers HOPX and FAM107A; pan-RG markers PAX6 and SOX2 in integrated scRNA-seq dataset; and anti-proliferative/pro-neurogenic marker BTG2 shows increased expression over time (left, day 93, and right, day 140) (n = 4 independent organoids). Scale bar (A), 200 μm. All scale bars in (B), 50 μm.