Figure 1.

Fetal hGPCs Can Be Converted to Induced DA Neurons In Vitro

(A and B) Fetal hGPCs visualized by bright field imaging (A) or stained for GFAP and PDGFRα (B).

(C) Reprogrammed TH⁺/TAU⁺ neurons 3 weeks after transgene activation.

(D and E) hGPCs kept in parallel for 3 weeks in neuronal conversion medium (D) or glial medium (E) do not give rise to any TH⁺/TAU⁺ neurons.

(F) qPCR analysis of reprogrammed cells 3 weeks after transgene activation shows an upregulation of pan-neuronal and DA genes.

(G) Representative trace of voltage responses from the whole-cell patch-clamp technique showing multiple induced APs.

(H) Representative trace of inward sodium- and outward potassium-rectifying currents triggered by stepwise depolarization of the cell.

(I and J) The reprogrammed fetal hGPCs show spontaneous postsynaptic currents (I) and spontaneous firing at their resting membrane potential (J).

In Figure 1F, data are presented as means ± SEM, and all data points have been visualized in the graphs. Each data point represents a replicate from an independent experiment (n = 5 for ALN + shREST; n = 2 for CNTRL-NDIFF; n = 3 for CNTRL-GM). Scale bars: (A, B, D, and E) 100 μm; (C) 50 μm. See also Figure S1.