Figure 3.

Direct Conversion of Purified CD140-expressing Progenitors

(A) Schematic overview of the strategy for neuronal conversion of CD140-sorted and unsorted cells side by side. Astrocyte biased progenitors are depicted in green, oligodendrocyte biased progenitors are depicted in pink, bipotent glial progenitors are depicted in purple, and cells of an unknown identity are depicted in blue.

(B and C) CD140-sorted hGPCs are positive for OLIG2/SOX10 (B) and PDGFRα (C). A subset of the hGPCs are positive for GFAP (B and C), representing a bipotent progenitor state.

(D and E) TH⁺/TAU⁺ cells 3 weeks after reprogramming, starting from either unsorted (neuronal proportion, 53.7%; TH+ neuronal proportion, 1.7%; D) or CD140-sorted (neuronal proportion, 57.0%; TH+ neuronal proportion, 1.3%; E) hESC-derived hGPCs.

Scale bars: (C) 100 μm; (B, D, and E) 50 μm. See also Figure S4.