Figure 6.

hESC-Derived hGPCs Reprogrammed iDANs Become Functionally Mature

(A) Graph showing the number of cells exhibiting multiple, single, or no AP responses evoked by current steps at 60 and 100 days after reprogramming start with ALN + shREST and ALN + Foxa2 + shREST.

(B) Representative trace of voltage responses from the whole-cell patch-clamp technique showing multiple induced APs in cells reprogrammed with ALN + shREST (left, purple) ALN + Foxa2 + shREST (right, green).

(C) Inward sodium current peak (left) and outward potassium current (right) plotted against voltage steps of cells reprogrammed with ALN + shREST (purple) and ALN + Foxa2 + shREST (green).

(D) Spontaneous postsynaptic currents in an ALN + shREST reprogrammed neuron.

(E) Spontaneous firing at resting membrane potential in an ALN + shREST reprogrammed neuron.

(F) Repetitive induced AP at small current injections in an ALN + shREST reprogrammed neuron.

See also Figure S6.