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. 2020 Oct 15;10:17378. doi: 10.1038/s41598-020-74281-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative methylation levels of α-amylase promoter regions measured by Chop-qPCR. (a) Map of PCR amplicons of CpG islands predicted by MethPrimer. (b) Relative DNA methylation levels of OsAmy1C, OsAmy3B, and OsAmy3E promoter regions in dry control and heat-stressed seeds. Significant differences are shown as P < 0.05* and P < 0.001*** according to Student’s t-test (n = 6). Error bars, SD.