Figure 5.

RELA Is Co-transactivated by Phosphorylation of AKT1 and MAPK3/1

(A) Quantitative real-time PCR analysis showed that miR-3613-5p upregulation was abrogated by treatment with MK-2206 2HCl, SCH772984, or MK-2206 2HCl and SCH772984 in RELA-overexpressing LUAD cells, normalized to U6. Mean ± SD, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, by Student’s t test. (B) Western blot analysis demonstrates the downregulation of p-AKT1, p-MAPK3/1, RELA, and JUN in RELA-overexpressing LUAD cells after MK-2206 2HCl, SCH772984, a combination of MK-2206 2HCl and SCH772984, or miR-3613-5p inhibitor treatment. GAPDH served as a NC.