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. 2020 Aug 28;9(9):1984. doi: 10.3390/cells9091984

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Elevated TMEM16A activity disturbs eNOS activation: (a) Noninduced nitric oxide levels and ACh-induced nitric oxide production of control (Ctrl^Ad) and TMEM16A-overexpressing (Ano1^Ad) human PAECs. Figures were generated with 8 sets of experiments with quadruplicate in each group and normalized to protein content. (b) Western blots showing ACh-induced changes in eNOS phosphorylation of Ctrl^Ad and Ano1^Ad-infected donor PAECs with quantification following the eNOS phosphorylation pattern at activatory Ser1177 and inhibitory Thr495 sites after 5, 15 and 30 min of ACh stimulation. (c) Quantification of basal, noninduced level of eNOS phosphorylation at Ser1177 and Thr495 as well as phosphorylation of Ser1177 15 min after ACh stimulation. Figures were generated with 6 samples. * p < 0.05, *** p < 0.001, ratio-paired t-test.