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. 2020 Sep 20;10(9):651. doi: 10.3390/brainsci10090651

Figure 6.

Figure 6

Glucose transporter 3 (GLUT3) expression is not increased in neurons after MCAO. Rats were subjected to middle cerebral artery occlusion (MCAO) during 2 h followed by restoration of blood flow (reperfusion) for 24 h. Control animals (CT) were subjected to simulated MCAO. At the onset of restoration of blood flow, animals received either vehicle, ethanol 50% (+VH) or resveratrol at 1.9 mg/kg, body weight (+RSV). Immunofluorescence was used to detect GLUT3 protein expression in neurons. 4′, 6-diamidino-2-phenylindole (DAPI) was used to detect nuclei. (A) Representative images show GLUT3 (green), microtubule-associated protein 2 (MAP2) (red), and nuclei (blue). NEG, Negative control without primary antibody. (B) Quantification of the fluorescence for GLUT3 expression in cells positive for the neuronal marker MAP2. Fluorescence was reported as R (Pearson’s correlation coefficient) of the signal associated with the colocalization of MAP2 and GLUT3 proteins in the region of interest (ROI). Values were expressed as mean ± standard deviation. ANOVA, Tukey, NS, not significant.