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Effect of Y/A319 and Y/A310 substitutions on RelA enzymatic activity. (A) Ratio of relative RelA activity of the induced to non-induced cells in an in vivo fluorescence assay. The induction with 1.5 µM IPTG took place at 210 min, indicated with the black triangle. (B) In vitro pppGpp production assay. Control is [γ-32P] ATP without enzyme.
