Figure 8.

Pimozide suppresses the growth of brain tumor cells by inducing STAT3-mediated autophagy. (A,B) Approximately 0.2 million Daoy and U-251 MG cells were transfected with 5 µg of STAT3 shRNA followed with the treatment of 15 µM pimozide for 48 h. STAT3 inhibition, the induction of autophagy marker LC3B, and the level of apoptosis induction were evaluated by Western blotting analysis. β-actin was used as a loading control. The figure is a representation of at least three independent experiments.