Figure 9.
Pimozide suppresses the growth of intracranially implanted brain tumor cells. (A) About 0.15 × 106 U-87MG luc cells were implanted intracranially in the brain of 4–6-week-old athymic nude mice. Mice were treated with 25 mg/kg pimozide starting day 14 after tumor cells were implanted until day 60. Representative luminescence (photons/second) was measured about thrice a week and plotted against days. The statistical significance level was considered as * P ≤ 0.05. (B) Representative mouse brain from the control and pimozide-treated groups after terminating the experiment. (C) After terminating the experiment, brains with tumors were removed aseptically, lysed, and analyzed for p-STAT3, STAT3, p-src, Mcl-1, Bcl-2, and LC3B. (D) Graphical representation of the protein expression in control and treatment groups when normalized with its respective β-actin.