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. 2020 Sep 4;10(9):1281. doi: 10.3390/biom10091281

Figure 3.

Figure 3

Negative regulation of VEGFA by miR-361-5p through direct 3′UTR binding. (A): The direct targeting of VEGFA by miR-361-5p confirmed by luciferase reporter assay in 293 cells (plus (+) indicates addition of oligos, minus (−) indicates no addition of oligos); (B,C): The mRNA levels of VEGFA after transfection of miR-361-5p mimics or inhibitors in porcine GCs; (D,E): The protein levels of VEGFA after transfection of miR-361-5p mimics or inhibitor in porcine GCs; (F,G): The levels of VEGFA after transfection of miR-361-5p mimics and inhibitors in culture media. n = 3 cell culture wells per group. Data are expressed as the mean ± SEM. Significant differences (p < 0.05) are indicated by different letters or * p < 0.05, ** p < 0.01.