Figure 5.

Transcription factor SMAD4 was involved in miR-361-5p mediated VEGFA expression. (A): Schematic diagram showing the genome location of the miR-361-5p coding gene and potential SMAD4 binding sites. (B): The promoter activity of MIR361 upstream region confirmed by the dual-luciferase reporter assay; (C,D): Overexpression and knockdown of SMAD4 weaken and enhanced MIR361 promoter activity, respectively; (E): The expression of miR-361-5p was not affected after SMAD4 overexpression; (F): The expression of miR-361-5p was up-regulated after SMAD4 knockdown; (G,H): The expression of VEGFA mRNA and protein levels were down-regulated after SMAD4 knockdown. n = 3 cell culture wells per group. Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01.