Figure 13.

Role of CD8⁺ T cells protection against L. infantum in LiChimera/Addavax vaccinated mice. The BALB/c mice were vaccinated twice intramuscularly with LiChimera adjuvanted with Addavax. Two weeks post boosting, mice were challenged with 1 × 10⁷ stationary phase L. infantum promastigotes. Mice were treated the day before L. infantum challenge and again on day 2 using anti-CD8⁺ monoclonal antibody. The control mice were treated with normal rat IgG. As the control for infection, PBS-injected mice were also included. The parasite load in liver (a) and spleen (b) was measured 8 weeks post challenge. Data are expressed as means ± s.d. for n = 4–5 mice per group. Significant differences between groups are indicated with asterisks measured by one-way ANOVA and Tukey’s multiple comparison tests. * p < 0.05, ** p < 0.01, and *** p < 0.001.