Table 2.
Primers used in this study.
| Primer Name | Primer Sequence (5′→3′) | PCR |
|---|---|---|
| BrkA U-F | gcctcttcgccaaagaagg | Fusion Ia, fusion II |
| BrkA U-R2 | GGAGCTCGCTCAGAAGCTGTgagaagttgaacaaaccgac | Fusion Ia |
| BrkA D-F2 | GTCGGTTTGTTCAACTTCTCacagcttctgagcgagctcc | Fusion Ib |
| BrkA D-R | agaaggcgtggttcctgg | Fusion Ib, II |
| BrkA check-F2 | ttcaggaaagctcttgttgg | Deletion check |
| BrkA check-R2 | cggcatggacttctaagtcc | Deletion check |
| Vag8 U-F | ctgcgtcaaccgcttagc | Fusion Ia, fusion II |
| Vag8U-R2 | GGTCACCAGCTGTAGCGATACctcaacacctcttggctag | Fusion Ia |
| Vag8D-F2 | CTAGCCAAGAGGTGTTGAGgtatcgctacagctggtgacc | Fusion Ib |
| Vag8-R | aagccgcgtgcgactacgtc | Fusion Ib, II |
| Vag8 check-F2 | ggcgttttctgtcaatcgtc | Deletion check |
| Vag8 check-R2 | gccgaactgcaacgctactg | Deletion check |