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. 2020 Sep 20;12(9):2686. doi: 10.3390/cancers12092686

Figure 4.

Figure 4

Figure 4

Inactivation of Furin in the mammary gland enhances apoptosis by impairing maturation of proIGF1R and proIR in TNBC mice. (A) Immunoblotting analysis of proIR and IR in tumor samples of multiparous PyMT;WT and PyMT;KO mice. Representative photo of protein levels of proIR and IR in tumor samples (left panel); quantification of IR/IR+proIR ratio in indicated tumor samples (right panel). (B) Immunoblotting analysis of proIGF1R and IGF1R in tumor samples of multiparous PyMT;WT and PyMT;KO mice. The protein levels in tumor samples are shown in the left panel; the quantification of IGF1R/IGF1R+proIGF1R ratio in indicated tumor samples in the right panel. (C) Inactivation of Furin in mammary epithelial cells reduces PI3K/AKT expression and signaling in TNBC tumor samples. Immunoblotting analysis of phosphorylated AKT and total AKT in indicated TNBC tumor samples (left panel). The quantification of pAKT and AKT ratio is indicated in the right panel. (D) Inactivation of Furin in mammary epithelial cells reduces MAPK/ERK1/2 expression and activity in TNBC tumor samples. Immunoblotting analysis of phosphorylated ERK1/2 and ERK1/2 in TNBC tumor samples is shown in the left panel and the quantification of pERK1/2 and ERK1/2 ratio is shown in the right panel. (E) Immunoblotting analysis of cleaved caspase 3 in TNBC tumor samples. Protein levels of cleaved caspase 3 in TNBC tumor samples (left panel) and quantitative analysis of cleaved caspase 3/GAPDH ratio in indicated tumor samples of TNBC mice (right panel). A two-tailed Student’s t-test was performed for A–E. * p < 0.05. ** p < 0.01. *** p < 0.001.