Figure 4.

Photodynamic therapy using talaporfin sodium (TS-PDT) causes phosphorylation of the myosin light chain (MLC) in human umbilical vein endothelial cells. The TS-PDT induction of MLC phosphorylation was investigated in vitro using western blot analysis. (a,b) MLC was used as a loading control and MLC phosphorylation was measured at various time points. (a) Western blot analysis and (b) quantification of the western blot bands. (c,d) MLC phosphorylation at 5 min post TS-PDT of different doses was measured and compared with that observed using CA4P. (c) Western blot analysis and (d) quantification of the western blot bands. One representative experiment out of three is shown here. The Whole Blots for Western Blot analysis for Figure 4a,c are shown in Figure S2a,b.