Figure 7.
Analysis of the microvessel density of HCT116 xenograft tumors treated with photodynamic therapy using talaporfin sodium (TS-PDT). (a) Histological sections of the control (laser-treated only) and TS-PDT-treated HCT116 tumors were stained for the detection of CD31 (original magnification, 200×; scale bar, 50 µm). One representative experiment out of five is shown here. (b) The mean area positive for CD31 (%), number of microvessels/mm2, and diameter of the microvessel (µm) in the viable tumors were determined through morphometric image analysis. Data are presented as the means ± SE from five independent experiments. The results were analyzed using Welch’s t-test; * p < 0.05 and ** p < 0.01.