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. 2020 Jul 22;8(3):403. doi: 10.3390/vaccines8030403

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The development of three different engineered human epidermal growth factor receptor 2 (HER2) antigens. (A) Diagram of the concept of engineered HER antigens based on the extracellular domain, transmembrane domain, and intracellular domain. (B) The gel image of each antigen. The K684, K965, or K1117 (from left to right, respectively) construct was inserted into an adenovirus shuttle vector and digested with restriction enzymes SalI and HindIII. The expression of engineered HER2 antigens on the THP-1 (C) and CD14+ monocytes (D) and CD20+ B cells (E) isolated from human peripheral blood (D) was analyzed by flow cytometry. All data are representative of two independent experiments.

The development of three different engineered human epidermal growth factor receptor 2 (HER2) antigens. (A) Diagram of the concept of engineered HER antigens based on the extracellular domain, transmembrane domain, and intracellular domain. (B) The gel image of each antigen. The K684, K965, or K1117 (from left to right, respectively) construct was inserted into an adenovirus shuttle vector and digested with restriction enzymes SalI and HindIII. The expression of engineered HER2 antigens on the THP-1 (C) and CD14+ monocytes (D) and CD20+ B cells (E) isolated from human peripheral blood (D) was analyzed by flow cytometry. All data are representative of two independent experiments.