Figure 2.

The analysis of the antibody response in the sera of vaccinated mice. (A,B) Naïve BALB/c mice were immunized with B cell and monocyte-based vaccine transduced by modified adenovirus carrying each antigen. One, 5, 7, 9, 11, and 13 weeks after immunization, antibodies in the mouse sera were titrated by flow cytometry (* p < 0.05, ** p < 0.01, *** p < 0.001 for K684 versus K1117, ^### p < 0.001, for K965 versus K1117). In vitro and in vivo cytotoxic T lymphocyte (CTL) analysis. Naïve BALB/c mice were immunized with B cell and monocyte-based vaccine transduced by modified adenovirus carrying each antigen. The splenocytes from immunized mice were co-cultured with attenuated CT26/HER2 cell lines on day 7. After 5 days CD8+ T cells were sorted by microbeads and cocultured with ⁵¹Cr-labeled P63 target peptide-loaded WEHI-164 cells (C). For in vivo CTL analysis, naïve BALB/c mice were immunized with B cell and monocyte-based vaccine transduced by modified adenovirus carrying each antigen. HER2 P63 peptide-loaded target cells and unloaded internal control cells labeled with CFSE high and low, respectively, were injected into immunized mice. One day after target cell injection, all mice were sacrificed, and specific cell lysis was analyzed with flow cytometry (D). (* p < 0.05, ** p < 0.01, *** p < 0.001).