Figure 7.

Validation of Hh signaling and PSMA at the mRNA level in DTX-resistant PCa cells. DTX-resistant (C4-2B-R and LNCaP-R) PCa cells were treated with or without A10-conjugated TQ-NP for 48 h. Levels of the indicated genes were measured in fold change relative to control cells by using RT-PCR. 18S rRNA was used as an internal control for data normalization. The experiments were repeated thrice. The data, presented as means ± SEM, were analyzed by Student’s t-test. The asterisks * and ** indicate p-values ≤ 0.05 and 0.01, respectively.