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. 2020 Aug 31;9(9):1999. doi: 10.3390/cells9091999

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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AI sheet groups showed better viability and glucose response than the islet-only group during the culture period. (A) The viability of islets and AI sheets was assessed by LIVE/DEAD staining. (B) Insulin release at 3.3 mM and 16.7 mM glucose in the hADSC sheet, islets, and AI sheet (in triplicate). The glucose-stimulated insulin secretion (GSIS) test showed better glucose response by the AI sheet group than by the islet-only group on days 1 and 4. Hundred hand-picked rat islets were used for each well. Results are presented as mean ± SD. Statistical significance was determined by a t-test of the stimulation index on days 1 and 4; * p < 0.05, n = 5.