Figure 4.

The liver and peritoneal surface were more effective sites for cell sheet transplantation among subcutaneous, liver surface, and peritoneal wall sites using human islets (5000 IEQ) and ADSC multi-layered sheets in diabetic nude mice (n = 4). (A) Transplantation of AI sheets onto the three sites was performed successfully. The liver and peritoneal surface were more effective for controlling blood glucose levels. (B) The liver surface and peritoneal wall groups showed increased body weight. The subcutaneous group-maintained body weight, but diabetic control mice dramatically decreased in weight. (C) Intraperitoneal glucose tolerance test (IPGTT). The mice were fasted for 8 h, and then 2 g glucose/kg body weight was injected intraperitoneally. The baseline blood glucose was measured at 0, 15, 30, 60, and 120 min. The blood glucose profile (C) and area under the curve (AUC) (D) during IPGTT in the peritoneal wall were similar to those of non-diabetic mice. The liver surface and peritoneal wall groups showed significantly controllable results compared to the subcutaneous site group. Values are expressed as means ± SD. (* p < 0.05). (E) H&E and immunohistochemical staining for insulin, CD31, and Ki67 at 3 weeks after transplantation of AI sheets in three different sites. (F) PKH26 red fluorescent cell linker-labeled insulinoma spheroid with ADSC sheet was examined after transplantation with an in vivo imaging system.