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. 2020 Aug 26;8(9):1301. doi: 10.3390/microorganisms8091301

Figure 1.

Figure 1

Specificity of selected bacterial species: (a) real-time LAMP assay (QuantStudio™ 6 Flex Real-Time PCR System); (b) gel electrophoresis of LAMP products confirms results of real-time LAMP; (c) Colorimetric Master Mix assay after 30 min reaction time; M—ladder 100 bp (NEB, Hitchin, UK), NC—negative control water, 1—Xanthomonas gardneri DSMZ 19127, 2—Xanthomonas axonopodis pv. vesicatoria CRI 1013, 3—Xanthomonas perforans DSMZ 18975, 4—Xanthomonas vesicatoria BCCM/LMG 920, 5—Erwinia amylovora CRI Ea10/96, 6—Burkholderia glumae BCCM/LMG 20138, 7—Clavibacter michiganensis susp. michiganensis CFBP 1460, 8—Clavibacter michiganensis subsp. sepedonicus NCPPB 3467, 9—Pseudomonas syringae pv. syringae NCPPB 2306, 10—Pseudomonas syringae pv. tomato CRI 8119, 11—Ralstonia solanacearum NCPPB 2505.