Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 20;11(9):965. doi: 10.3390/genes11090965

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Novel cassettes are effective for allelic replacement of capsule. Cassettes (below column) replaced the capsule loci in (a) TIGR4/TIGR4S and (b) D39/D39S. The acaspular mutants were transformed with D39 genomic DNA and transformants were selected on counter-selection agents listed in Table 5, with the concentration of chlorinated phenylamine ranging from 7.5 mM to 15 mM. The number of true-positive colonies (red bars, number above column) and the number of false-positive colonies (black bars) were determined per mL of transformation. Dashed line represents upper limit of detection.