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. 2020 Aug 20;11(9):965. doi: 10.3390/genes11090965

Table 5.

Selective agents used in TSA (tryptic soy agar) plates when replacing the markerless cassette in step two transformation.

Markerless Cassette Counter-Selection Agents
Sweet Janus 800 µg/mL Streptomycin + 10% Sucrose
NewSweet Janus 800 µg/mL Streptomycin + 10% Sucrose
Phun Janus 800 µg/mL Streptomycin + 7.5–15 mM Chlorinated-Phenylalanine
PhunSweet Janus 800 µg/mL Streptomycin + 10% Sucrose + 7.5–15 mM Chlorinated-Phenylalanine
PhunSweet 10% Sucrose + 7.5–15 mM Chlorinated-Phenylalanine
Phun 7.5–15 mM Chlorinated-Phenylalanine