Table 7.
Ratio of true positive to false positive colonies of TIGR4SΔcps::NewSweetJanus and D39SΔcps::NewSweetJanus transformed with genomic DNA of different serotypes. NSJ refers to NewSweetJanus. Selection occurred on 800 µg/mL streptomycin (Strep), 10% sucrose (Suc), or 7.5–15 mM chlorinated-phenylalanine (Chl-Phe).
| Strain | gDNA | Selection | Ratio |
|---|---|---|---|
| TIGR4SΔcps::NSJ | TIGR4 (4) | Strep, Suc | 0.08 |
| TIGR4SΔcps::NSJ | D39 (2) | Strep, Suc | 1.33 |
| TIGR4SΔcps::NSJ | ABCA38 (22F) | Strep, Suc | 0 |
| TIGR4SΔcps::NSJ | CDC007 (6B) | Strep, Suc | 0.11 |
| TIGR4SΔcps::NSJ | ABCA31 (35B) | Strep, Suc | 0.07 |
| TIGR4SΔcps::NSJ | 45 | Strep, Suc | 0.10 |
| TIGR4SΔcps::NSJ | CDC001 (9V) | Strep, Suc | 0.04 |
| TIGR4SΔcps::NSJ | CDC004 (23F) | Strep, Suc | 0.09 |
| TIGR4SΔcps::NSJ | DAW4 (11) | Strep, Suc | 0.35 |
| TIGR4SΔcps::NSJ | ABCA61 (3) | Strep, Suc | 0 |
| TIGR4SΔcps::NSJ | CDC030 (12E) | Strep, Suc | 0 |
| TIGR4SΔcps::NSJ | CDC048 (18C) | Strep, Suc | 0.19 |
| TIGR4SΔcps::NSJ | CDC009 (15C) | Strep, Suc | 0.19 |
| D39SΔcps::NSJ | TIGR4 (4) | Strep, Suc | 0.02 |
| D39SΔcps::NSJ | D39 (2) | Strep, Suc | 1.14 |
| D39SΔcps::NSJ | ABCA38 (22F) | Strep, Suc | 0.02 |
| D39SΔcps::NSJ | CDC007 (6B) | Strep, Suc | 0.06 |
| D39SΔcps::NSJ | ABCA31 (35B) | Strep, Suc | 0.36 |
| D39SΔcps::NSJ | 45 | Strep, Suc | 0 |
| D39SΔcps::NSJ | CDC001 (9V) | Strep, Suc | 0.04 |
| D39SΔcps::NSJ | CDC004 (23F) | Strep, Suc | 0.07 |
| D39SΔcps::NSJ | DAW4 (11) | Strep, Suc | 0.20 |
| D39SΔcps::NSJ | ABCA61 (3) | Strep, Suc | 0.03 |
| D39SΔcps::NSJ | CDC030 (12E) | Strep, Suc | 0 |
| D39SΔcps::NSJ | CDC048 (18C) | Strep, Suc | 0.05 |
| D39SΔcps::NSJ | CDC009 (15C) | Strep, Suc | 0.09 |