Figure 3.

Altered expression of microglial endocannabinoid system genes during EAE. (A) Flow cytometry gating strategy for the analysis of microglial cells from EAE mice. Microglia were sorted as CD11b⁺ cells with low CD45 expression. (B–D) RT-qPCR analysis of endocannabinoid signaling genes in microglia purified at presymptomatic (B), acute (C), and recovery (D) phases of EAE. Fold changes in relative expression compared to naïve microglia were determined as log₂ (n = 5–6 mice group). Changes in relative expression were determined as log₂. N.D., not detected. * p < 0.05; ** p < 0.01; *** p < 0.001; referred to naïve astrocytes by Student´s t-tests or Mann–Whitney U-tests.