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. 2020 Jun 30;8(3):349. doi: 10.3390/vaccines8030349

Figure 5.

Figure 5

BiFC analysis between GmSNAP18, GmPR08, and the nine GmSHMT08 mutated alleles. The coding sequence of the 9 GmSHMT08 mutant alleles were cloned into pSAT4-nEYFP-C1 to generate nEYFP-GmSHMT08 mutant fusions. Likewise, GmSNAP18 from the Forrest WT and GmPR08-Bet VI were cloned into pSAT4-cEYFP-C1-B and pG2RNAi2 to generate cEYFP-GmSNAP18 and pG2RNAi2-GmPR08-Bet VI fusions. Various combinations of cEYFP and nEYFP control fusions were co-expressed in onion epidermal cells by particle bombardment (Figure S2). Bar = 200 µM.