Figure 7.

LC-MS protein identification of the eluted fraction obtained by immunoprecipitation using immobilized anti-SHMT08 antibodies and expression analysis. (A) Fragmented peptides identified by LC-MS in SCN infected root samples from Forrest. (B) Alignment of the GmPRXD16 protein sequence showing the five identified fragmented peptides by LC-MS. (C) Expression analysis of component of the ROS signaling pathway reveals that both genes are co-regulated in root cells undergoing nematode infection. Transcripts of genes encoding key components of the ROS signaling pathway including the peroxidase GmPRXD16 and the protein kinase GmPKR19 genes were induced and more abundant under SCN infection in the resistant line Forrest than in the susceptible line Essex. Asterisks indicate significant differences between the tested lines as determined by ANOVA (*** p < 0.001, ** p < 0.01, * p < 0.05).