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. 2020 Aug 26;9(9):1975. doi: 10.3390/cells9091975

Figure 3.

Figure 3

(A) Representative histograms of NK cell degranulation towards the Beas-2B cell target. NK cells were marked with CD56 and gated as reported in Figure S1C. The degranulation was assessed by CD107a-PE expression. Beas-2B was transfected with SP1, SP2, and S proteins or control fluorescent antibody was used as the positive control (null-transfected), or treated with GATA3 inhibitor (anti-GATA). (B) MFI of CD107a-PE-positive NK cells after the co-culture with Beas-2B cells. (C) CFSE+/7-AAD+ cell percentage in NK cell/Beas-2B co-cultures. Beas-2B alone and treated with Triton-x100 (0.8%) were used as negative and positive controls, respectively. The values are presented as mean ± standard deviation. (D) Representative histograms of HLA-I expression on the surface of transfected Beas-2B. (E) The histograms showed the mean ± standard deviation MFI (mean fluorescence intensity) values of HLA-I expression in three independent experiments. * significant p values Student t test; (F) Representative histograms of HLA-E expression on the surface of transfected Beas-2B cells. (G) The histograms showed the mean ± standard deviation MFI (mean fluorescence intensity) values of HLA-E expression in three independent experiments. * significant p values t test. (H) Relative ratio of Real-Time PCR of HLA-E expression in transfected Beas-2B cells. SP1 transfected Beas-2B cells were also treated with GATA inhibitor (anti-GATA). * significant p values Student t test.