. 2020 Sep 9;10(9):1302. doi: 10.3390/biom10091302

Table 1.

Multiple reaction monitoring (MRM) parameters: precursor and product ion transitions (quantifier underlined) for all the analytes and internal standards (ISs), de-clustering potential (DP), and collision energy (CE).

Compound	Precursor Ion (m/z)	Product Ions (m/z)	DP (eV)	CE (eV)
AA (20:4)	303.1	59.1	−45	−42
		259.6	−45	−20
EPA (20:5)	301.4	59.1	−55	−42
		203.1	−55	−20
DHA (22:6)	327.3	283.3	−80	−10
		59.1	−80	−35
TXB₂	369	177	−50	−22
		195	−50	−20
PGE₂	351.5	315	−50	−25
		271.1	−50	−25
PGD₂	351.5	271	−50	−30
		189	−50	−30
PGF_2α	353	291	−50	−35
		193	−50	−35
6αKeto-PGF_1α	369.5	245	−50	−35
		163	−50	−35
LTB₄	335	273	−45	−23
		195	−45	−23
5(S)-HETE	319.5	115	−50	−18
		301.1	−50	−18
15(S)-HETE	319.5	219	−50	−15
		301.2	−50	−15
14,15-EET	319.,5	219.1	−50	−22
		301	−50	−40
AEA	348	62	76	42
		133	76	33
2AG	379.4	287.3	76	18
		203	76	25
LNEA	322.3	62.2	85	35
		81.2	85	35
LEA	324.3	62.2	85	35
		109	85	32
PEA	300.1	62	98	19
		283	98	36
OEA	326.3	62.2	85	35
		309	85	21
SEA	328.3	62.2	85	35
		311.1	85	22
DHEA	372.3	62	85	18
		67	85	36
AGly	362.3	287	85	18
		76	85	18
ADA	440.5	137	95	34
		154	95	23
2AGE	365.3	273	85	10
		121	85	20
ODA	418.3	137	85	24
		154	85	35
EPEA	346.3	62	85	35
		135	85	35
ASer	392.5	106	85	35
		137.3	85	33
OGly	340.5	76	85	35
		265	85	35
PalGly	314.5	76	85	35
		239	85	20
AGABA	406.5	287.4	85	24
		84.1	85	55
A5HT	463.3	160.4	85	35
		132.2	85	35
O5HT	441.7	160.4	85	35
		132.2	85	35
Pal5HT	415.7	160.4	130	47
		132.2	130	47
TXB₂-d4	373	199	−50	−22
		173	−50	−22
PGF_2a-d4	357	295	−50	−35
		197	−50	−35
LTB₄-d4	339	197	−45	−23
		277	−45	−23
EPA-d5	306.3	59.1	−50	−35
		208.1	−50	−18
AEA-d8	356.3	62	76	35
		70	76	35
SEA-d4	332.3	66.2	85	35
		62	85	18
EPEA-d4	350.3	66	85	35
		135	85	35
OEA-d2	328.3	62	85	35
		311	85	35
PEA-d5	305.1	62	85	35
		288	85	35
ADA-d8	448.5	137	85	35
		154	85	35
AGly-d8	370.6	76	85	20
		84	85	20
ASer-d8	400.6	106	85	35
		70	85	35
O5HT-d17	458.7	160.4	130	47
		132.2	130	47

Abbreviations: arachidonoyl acid (AA), eicosapentaenoyl acid (EPA), docosahexaenoic acid (DHA), thromboxane-B2 (TXB2), prostaglandin-F2α (PGF2α), 6α-keto-prostaglandin-F1α (6α-keto-PGF1α), prostaglandin-E2 (PGE2), prostaglandin-D2 (PGD2), leukotriene-B4 (LTB4), 5-hydroxyeicosatetraenoic acid (5(S)-HETE), 15-hydroxyeicosatetraenoic acid (15(S)-HETE), (±)14(15)-epoxyeicosatrienoic acid (14,15-EET), arachidonoylethanolamide (AEA), N-linolenoylethanolamide (LNEA), N-linoleoylethanolamide (LEA), N-oleoylethanolamide (OEA), N-palmitoylethanolammide (PEA), N-stearoylethanolamide (SEA), N-docosahexaenoylethanolamide (DHEA), N-eicosapentaenoylethanolamide (EPEA), N-arachidonoyldopamine (ADA), N-oleoyldopamine (ODA), N-arachidonoylglycine (AGly), N-oleoylglycine (OGly), N-palmitoylglicine (PalGly), N-arachidonoylserine (ASer), N-arachidonoylserotonine (A5HT), N-oleoylserotonine (O5HT), N-palmitoylserotonine (Pal5HT), 2-arachidonoylglycerylether (2AGE), 2-arachidonoyglycerol (2AG), N-arachidonoyl-3-hydroxy-γ-aminobutyric acid (AGABA), eicosapentaenoyl acid-d5 (EPA-d5), thromboxane-B2-d4 (TXB2-d4), prostaglandin-F2α-d4 (PGF2α-d4), and leukotriene-B4-d4 (LTB4-d4), N-arachidonoylethanolamide-d8 (AEA-d8), N-oleoylethanolamide-d2 (OEA-d2), N-palmitoylethanolammide-d5 (PEA-d5), N-stearoylethanolamide-d4 (SEA-d4), N-eicosapentaenoylethanolamide-d4 (EPEA-d4), N-arachidonoyldopamine-d8 (ADA-d8), N-arachidonoylglycine-d8 (AGly-d8), N-arachidonoylserine-d8 (ASer-d8), N-oleoylserotonine-d17 (O5HT-d17).