Table 2.
cELISA results for each single test performed. n.d., not detectable; St. Dev, standard deviation; SE, standard error; CV, coefficient of variation.
| Operator | Day | OAg (µg/mL) | St. Dev (µg/mL) | CV % | SE | SE % | Accuracy % | |
|---|---|---|---|---|---|---|---|---|
| Determination of S. sonnei OAg in single component DP supernatant | A | 1 | 1.60 | 0.19 | 11.67 | 0.031 | 1.945 | 95.97 |
| A | 1 | 1.75 | 0.18 | 10.08 | 0.029 | 1.680 | 104.64 | |
| A | 1 | 1.76 | 0.22 | 12.46 | 0.037 | 2.077 | 105.35 | |
| A | 1 | 1.71 | 0.32 | 18.51 | 0.053 | 3.085 | 102.61 | |
| A | 1 | 1.66 | 0.31 | 18.66 | 0.052 | 3.111 | 99.49 | |
| A | 2 | 1.69 | 0.26 | 15.17 | 0.043 | 2.528 | 101.23 | |
| A | 2 | 1.74 | 0.12 | 7.03 | 0.020 | 1.172 | 104.22 | |
| A | 2 | 1.74 | 0.14 | 8.28 | 0.024 | 1.381 | 103.97 | |
| A | 2 | 1.82 | 0.36 | 19.66 | 0.060 | 3.277 | 108.76 | |
| A | 2 | 1.80 | 0.35 | 19.55 | 0.059 | 3.259 | 108.01 | |
| A | 3 | 1.58 | 0.18 | 11.63 | 0.031 | 1.939 | 94.63 | |
| A | 3 | 1.65 | 0.19 | 11.37 | 0.031 | 1.896 | 99.08 | |
| A | 3 | 1.72 | 0.15 | 8.47 | 0.024 | 1.412 | 103.31 | |
| A | 3 | 1.74 | 0.14 | 7.87 | 0.023 | 1.343 | 104.22 | |
| A | 3 | 1.64 | 0.30 | 18.10 | 0.050 | 3.016 | 98.48 | |
| B | 1 | 1.58 | 0.16 | 10.09 | 0.027 | 1.682 | 94.89 | |
| B | 1 | 1.67 | 0.17 | 10.36 | 0.029 | 1.726 | 100.11 | |
| B | 1 | 1.73 | 0.14 | 8.19 | 0.024 | 1.365 | 103.57 | |
| B | 1 | 1.73 | 0.31 | 18.02 | 0.052 | 3.003 | 103.91 | |
| B | 1 | 1.73 | 0.31 | 17.75 | 0.051 | 2.959 | 103.68 | |
| B | 2 | 1.44 | 0.22 | 15.03 | 0.036 | 2.506 | 86.26 | |
| B | 2 | 1.60 | 0.18 | 11.22 | 0.030 | 1.871 | 95.80 | |
| B | 2 | 1.64 | 0.18 | 11.18 | 0.031 | 1.863 | 98.19 | |
| B | 2 | 1.71 | 0.33 | 19.19 | 0.055 | 3.198 | 102.22 | |
| B | 2 | 1.71 | 0.33 | 19.32 | 0.055 | 3.219 | 102.36 | |
| B | 3 | 1.62 | 0.23 | 14.39 | 0.039 | 2.399 | 96.82 | |
| B | 3 | 1.61 | 0.13 | 8.09 | 0.022 | 1.348 | 96.41 | |
| B | 3 | 1.58 | 0.15 | 9.35 | 0.025 | 1.558 | 94.88 | |
| B | 3 | 1.57 | 0.30 | 19.15 | 0.050 | 3.192 | 94.03 | |
| B | 3 | 1.55 | 0.30 | 19.64 | 0.051 | 3.274 | 92.91 | |
| Determination of S. sonnei OAg on multicomponent DP supernatant | A | 1 | 0.35 | 0.05 | 14.29 | 0.008 | 2.382 | 107.84 |
| A | 1 | 0.35 | 0.04 | 10.89 | 0.006 | 1.815 | 108.80 | |
| A | 1 | 0.35 | 0.03 | 7.28 | 0.004 | 1.248 | 109.72 | |
| A | 1 | 0.34 | 0.03 | 8.47 | 0.005 | 1.474 | 106.41 | |
| A | 1 | 0.34 | 0.04 | 12.37 | 0.008 | 2.337 | 104.84 | |
| A | 2 | 0.35 | 0.02 | 6.49 | 0.004 | 1.082 | 108.61 | |
| A | 2 | 0.36 | 0.03 | 7.06 | 0.004 | 1.176 | 112.31 | |
| A | 2 | 0.35 | 0.03 | 7.49 | 0.004 | 1.285 | 108.89 | |
| A | 2 | 0.35 | 0.02 | 6.36 | 0.004 | 1.107 | 108.55 | |
| A | 2 | 0.34 | 0.02 | 6.00 | 0.004 | 1.134 | 106.06 | |
| A | 3 | 0.30 | 0.03 | 10.61 | 0.005 | 1.768 | 93.29 | |
| A | 3 | 0.31 | 0.02 | 7.05 | 0.004 | 1.176 | 95.22 | |
| A | 3 | 0.30 | 0.03 | 9.73 | 0.005 | 1.669 | 94.26 | |
| A | 3 | 0.30 | 0.03 | 10.97 | 0.006 | 1.910 | 93.36 | |
| A | 3 | 0.30 | 0.03 | 8.71 | 0.005 | 1.646 | 94.89 | |
| B | 1 | 0.30 | 0.03 | 11.11 | 0.006 | 1.851 | 94.59 | |
| B | 1 | 0.31 | 0.04 | 13.52 | 0.007 | 2.253 | 97.28 | |
| B | 1 | 0.31 | 0.04 | 12.16 | 0.006 | 2.085 | 96.93 | |
| B | 1 | 0.31 | 0.10 | 30.77 | 0.017 | 5.356 | 97.69 | |
| B | 1 | 0.31 | 0.03 | 8.94 | 0.005 | 1.690 | 96.16 | |
| B | 2 | 0.31 | 0.02 | 5.92 | 0.003 | 0.986 | 97.23 | |
| B | 2 | 0.33 | 0.02 | 6.38 | 0.003 | 1.063 | 101.45 | |
| B | 2 | 0.32 | 0.02 | 7.52 | 0.004 | 1.289 | 100.11 | |
| B | 2 | 0.32 | 0.09 | 28.60 | 0.016 | 4.979 | 98.95 | |
| B | 2 | 0.32 | 0.03 | 8.95 | 0.005 | 1.692 | 99.90 | |
| B | 3 | 0.34 | 0.05 | 14.19 | 0.008 | 2.365 | 105.10 | |
| B | 3 | 0.28 | 0.03 | 10.03 | 0.005 | 1.672 | 88.59 | |
| B | 3 | 0.29 | 0.05 | 18.89 | 0.009 | 3.240 | 89.02 | |
| B | 3 | 0.30 | 0.09 | 31.41 | 0.016 | 5.468 | 93.48 | |
| B | 3 | 0.29 | 0.04 | 14.58 | 0.008 | 2.756 | 90.47 | |
| N1 | A | 1 | n.d | --- | --- | --- | --- | --- |
| A | 2 | n.d | --- | --- | --- | --- | --- | |
| A | 3 | n.d | --- | --- | --- | --- | --- | |
| B | 1 | n.d | --- | --- | --- | --- | --- | |
| B | 2 | n.d | --- | --- | --- | --- | --- | |
| B | 3 | n.d | --- | --- | --- | --- | --- | |
| N2 | A | 1 | n.d | --- | --- | --- | --- | --- |
| A | 2 | n.d | --- | --- | --- | --- | --- | |
| A | 3 | n.d | --- | --- | --- | --- | --- | |
| B | 1 | n.d | --- | --- | --- | --- | --- | |
| B | 2 | n.d | --- | --- | --- | --- | --- | |
| B | 3 | n.d | --- | --- | --- | --- | --- | |
| N3 | A | 1 | n.d | --- | --- | --- | --- | --- |
| A | 2 | n.d | --- | --- | --- | --- | --- | |
| A | 3 | n.d | --- | --- | --- | --- | --- | |
| B | 1 | n.d | --- | --- | --- | --- | --- | |
| B | 2 | n.d | --- | --- | --- | --- | --- | |
| B | 3 | n.d | --- | --- | --- | --- | --- |