Table 3.
FAcE assay results for each single test performed. n.d., not detectable; St. Dev, standard deviation; SE, standard error; CV, coefficient of variation.
| Operator | Day | OAg (µg/mL] | St. Dev (µg/mL) | CV % | SE | SE % | Accuracy % | |
|---|---|---|---|---|---|---|---|---|
| Determination of S. sonnei OAg in single component DP supernatant | A | 1 | 17.73 | 2.98 | 16.82 | 0.497 | 2.803 | 101.44 |
| A | 1 | 14.77 | 1.84 | 12.48 | 0.307 | 2.079 | 84.50 | |
| A | 1 | 15.71 | 1.88 | 11.94 | 0.322 | 2.047 | 89.91 | |
| A | 1 | 17.88 | 2.08 | 11.61 | 0.361 | 2.021 | 102.30 | |
| A | 1 | 15.77 | 3.96 | 25.10 | 0.748 | 4.743 | 90.23 | |
| A | 2 | 16.83 | 1.40 | 8.30 | 0.233 | 1.383 | 96.32 | |
| A | 2 | 18.23 | 4.12 | 22.59 | 0.740 | 4.058 | 104.32 | |
| A | 2 | 19.04 | 1.93 | 10.15 | 0.322 | 1.691 | 108.95 | |
| A | 2 | 21.36 | 3.14 | 14.70 | 0.523 | 2.449 | 122.24 | |
| A | 2 | 18.56 | 2.89 | 15.54 | 0.481 | 2.591 | 106.22 | |
| A | 3 | 17.59 | 2.05 | 11.64 | 0.362 | 2.059 | 100.68 | |
| A | 3 | 17.74 | 3.22 | 18.14 | 0.569 | 3.206 | 101.52 | |
| A | 3 | 19.03 | 1.91 | 10.06 | 0.338 | 1.778 | 108.88 | |
| A | 3 | 18.47 | 2.69 | 14.58 | 0.476 | 2.578 | 105.69 | |
| A | 3 | 18.74 | 2.16 | 11.54 | 0.382 | 2.040 | 107.27 | |
| B | 1 | 15.46 | 2.54 | 16.43 | 0.423 | 2.738 | 88.46 | |
| B | 1 | 17.09 | 1.62 | 9.50 | 0.271 | 1.584 | 97.78 | |
| B | 1 | 17.85 | 2.34 | 13.13 | 0.390 | 2.188 | 102.12 | |
| B | 1 | 17.25 | 2.30 | 13.32 | 0.388 | 2.251 | 98.72 | |
| B | 1 | 18.47 | 3.13 | 16.94 | 0.521 | 2.823 | 105.68 | |
| B | 2 | 17.25 | 3.49 | 20.21 | 0.581 | 3.369 | 98.69 | |
| B | 2 | 19.90 | 3.00 | 15.06 | 0.499 | 2.509 | 113.89 | |
| B | 2 | 19.09 | 3.25 | 17.03 | 0.542 | 2.838 | 109.24 | |
| B | 2 | 18.67 | 2.46 | 13.15 | 0.415 | 2.223 | 106.81 | |
| B | 2 | 18.02 | 1.71 | 9.50 | 0.289 | 1.605 | 103.11 | |
| B | 3 | 17.02 | 3.87 | 22.76 | 0.732 | 4.302 | 97.39 | |
| B | 3 | 15.05 | 2.33 | 15.48 | 0.440 | 2.925 | 86.15 | |
| B | 3 | 15.47 | 2.14 | 13.80 | 0.404 | 2.608 | 88.55 | |
| B | 3 | 15.40 | 2.44 | 15.85 | 0.470 | 3.051 | 88.15 | |
| B | 3 | 14.82 | 1.72 | 11.62 | 0.331 | 2.236 | 84.79 | |
| Determination of S. sonnei OAg on multicomponent DP supernatant | A | 1 | 4.62 | 0.54 | 11.74 | 0.090 | 1.957 | 93.39 |
| A | 1 | 4.55 | 0.54 | 11.80 | 0.092 | 2.023 | 91.89 | |
| A | 1 | 4.70 | 0.36 | 7.55 | 0.062 | 1.315 | 95.05 | |
| A | 1 | 4.61 | 0.51 | 11.07 | 0.096 | 2.091 | 93.28 | |
| A | 1 | 4.57 | 0.51 | 11.09 | 0.084 | 1.848 | 92.29 | |
| A | 2 | 4.77 | 0.73 | 15.24 | 0.125 | 2.613 | 96.51 | |
| A | 2 | 4.62 | 0.65 | 14.12 | 0.114 | 2.457 | 93.47 | |
| A | 2 | 4.70 | 0.61 | 13.05 | 0.116 | 2.466 | 94.97 | |
| A | 2 | 4.73 | 0.58 | 12.29 | 0.097 | 2.048 | 95.56 | |
| A | 2 | 4.75 | 0.48 | 10.03 | 0.082 | 1.721 | 95.99 | |
| A | 3 | 4.85 | 0.70 | 14.42 | 0.122 | 2.509 | 98.06 | |
| A | 3 | 5.05 | 0.73 | 14.40 | 0.138 | 2.721 | 102.19 | |
| A | 3 | 5.47 | 0.49 | 8.87 | 0.081 | 1.478 | 110.67 | |
| A | 3 | 5.46 | 0.61 | 11.16 | 0.105 | 1.914 | 110.47 | |
| A | 3 | 5.46 | 0.73 | 13.28 | 0.126 | 2.312 | 110.42 | |
| B | 1 | 5.25 | 0.65 | 12.30 | 0.122 | 2.325 | 106.04 | |
| B | 1 | 5.46 | 0.67 | 12.29 | 0.112 | 2.049 | 110.45 | |
| B | 1 | 5.41 | 0.81 | 15.04 | 0.140 | 2.579 | 109.43 | |
| B | 1 | 5.22 | 0.78 | 14.94 | 0.136 | 2.600 | 105.52 | |
| B | 1 | 5.15 | 0.77 | 15.04 | 0.146 | 2.842 | 104.14 | |
| B | 2 | 4.84 | 0.33 | 6.83 | 0.055 | 1.138 | 97.75 | |
| B | 2 | 4.98 | 0.47 | 9.50 | 0.081 | 1.630 | 100.67 | |
| B | 2 | 4.69 | 0.53 | 11.24 | 0.092 | 1.956 | 94.72 | |
| B | 2 | 4.80 | 0.52 | 10.82 | 0.098 | 2.045 | 97.09 | |
| B | 2 | 12.51 | 1.16 | 9.27 | 0.193 | 1.544 | 108.52 | |
| B | 3 | 11.50 | 1.02 | 8.88 | 0.170 | 1.480 | 99.79 | |
| B | 3 | 10.60 | 1.48 | 14.00 | 0.247 | 2.333 | 91.98 | |
| B | 3 | 12.4 | 1.18 | 9.49 | 0.196 | 1.582 | 107.60 | |
| B | 3 | 11.16 | 1.86 | 16.66 | 0.310 | 2.777 | 96.84 | |
| B | 3 | 10.98 | 1.07 | 9.73 | 0.178 | 1.622 | 95.28 | |
| Positive control (single component formulations) | A | 1 | 12.51 | 1.16 | 9,.27 | 0.193 | 1.54 | 108.52 |
| A | 2 | 11.5 | 1.02 | 8.88 | 0.170 | 1.48 | 99.79 | |
| A | 3 | 10.6 | 1.48 | 14.00 | 0.247 | 2.33 | 91.98 | |
| B | 1 | 12.4 | 1.18 | 9.49 | 0.196 | 1.58 | 107.60 | |
| B | 2 | 11.16 | 1.86 | 16.66 | 0.310 | 2.77 | 96.84 | |
| B | 3 | 10.98 | 1.07 | 9.73 | 0.178 | 1.62 | 95.28 | |
| N1 | A | 1 | n.d | --- | --- | --- | --- | --- |
| A | 2 | n.d | --- | --- | --- | --- | --- | |
| A | 3 | n.d | --- | --- | --- | --- | --- | |
| B | 1 | n.d | --- | --- | --- | --- | --- | |
| B | 2 | n.d | --- | --- | --- | --- | --- | |
| B | 3 | n.d | --- | --- | --- | --- | --- | |
| N2 | A | 1 | n.d | --- | --- | --- | --- | --- |
| A | 2 | n.d | --- | --- | --- | --- | --- | |
| A | 3 | n.d | --- | --- | --- | --- | --- | |
| B | 1 | n.d | --- | --- | --- | --- | --- | |
| B | 2 | n.d | --- | --- | --- | --- | --- | |
| B | 3 | n.d | --- | --- | --- | --- | --- | |
| N3 | A | 1 | n.d | --- | --- | --- | --- | --- |
| A | 2 | n.d | --- | --- | --- | --- | --- | |
| A | 3 | n.d | --- | --- | --- | --- | --- | |
| B | 1 | n.d | --- | --- | --- | --- | --- | |
| B | 2 | n.d | --- | --- | --- | --- | --- | |
| B | 3 | n.d | --- | --- | --- | --- | --- |