Table 3.
Number of differentially expressed genes (DEGs) found in both—pathways and DEGs of control transgenic samples (p < 0.0001) 1.
| Number of Genes in Overlap | |||
|---|---|---|---|
| WT vs. G232E | WT vs. R482L | R482L vs. G232E | |
| Myogenesis (Hallmark database) | 29 | 15 | 14 |
| Muscle structure development | 54 | 16 | 23 |
| Muscle system process | 48 | 20 | 24 |
| Muscle contraction | 41 | 18 | 21 |
| Striated muscle cell differentiation | 38 | 11 | 18 |
| Muscle cell differentiation | 41 | 11 | 19 |
| Muscle cell development | 30 | 10 | 14 |
| Myofibril assembly | 20 | 8 | 11 |
| Cellular component assembly involved in morphogenesis | 20 | 8 | 11 |
| Striated muscle contraction | 20 | 10 | 10 |
| Anatomical structure formation involved in morphogenesis | 39 | 14 | 20 |
| Muscle filament sliding | 13 | 8 | 7 |
| Actomyosin structure organization | 20 | 8 | 11 |
| Actin filament based process | 31 | ns | 15 |
| Sarcomere organization | 13 | ns | 7 |
| Myotube differentiation | 16 | ns | 9 |
| Muscle fiber development | 14 | ns | 9 |
| Actin mediated cell contraction | ns | 8 | ns |
| Regulation of muscle contraction | ns | 7 | ns |
1 Pathway analysis was performed on upregulated DEGs in WT/G232E, WT/R482L, and R482L/G232E pairs and combined into one table that represent upregulated pathways. GO biological processes and hallmark databases were used for pathway analysis with false discovery rate 0.05.