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Release of HLA-class Ib molecules by hAECs. (A) Soluble (s) HLA-G and -E molecules were quantified by ELISA in supernatants from 6 different batches of hAECs, collected after 24 h (white columns) or 72 h (grey columns) of culture. Results are expressed as ng/mL (sHLA-G) or units/mL (sHLA-E). (B) sHLA-G1/G5 and sHLA-G5 were quantified by ELISA in 4 random different batches of hAEC using MEM-G9 or 5A6G7 mAbs as a capture reagent, respectively.
