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. 2020 Aug 20;9(9):1928. doi: 10.3390/cells9091928

Figure 2.

Figure 2

TonEBP promotes autophagy in pancreatic β cells. (A) MIN6-M9 cells transfected with scrambled siRNA (scr) or TonEBP-targeted siRNA (Ton) were treated for 6 h with vehicle (VH), brefeldin A (BFA; 20 μM), or tunicamycin (TM; 1 μg/mL). TonEBP, LC3-II, and Hsc70 were immunoblotted. (B) Cells transfected and treated as above were immunostained for LC3. (C) Percent of LC3 positive cells and LC3 signal intensity was measured in 150 cells from each group from (B). (D,E) Cells transfected with siRNA as above were pre-treated for 1 h with chloroquine (CQ; 10 μM) or LY294002 (LY; 10 μM) followed by a 4 h treatment with TM (1 μg/mL). (D) Cells were immunostained for LC3. (E) Percent of LC3 positive cells and LC3 signal intensity was measured in 50 cells from each group. Mean + SD. # p < 0.05 vs. scrambled siRNA-VH. * p < 0.05. Scale bars, 50 μm (B,D).